Journal: BMC Cancer

Article Title: 3- O -Acetyloleanolic acid inhibits VEGF-A-induced lymphangiogenesis and lymph node metastasis in an oral cancer sentinel lymph node animal model

doi: 10.1186/s12885-018-4630-0

Figure Lengend Snippet: Effects of 3AOA on expressions of VEGFR-1 and VEGFR-2, and activation of VEGFR-1, VEGFR-2 and lymphangiogenesis related downstream signaling factors in rhVEGF-A-treated HLMECs. a-b Expression levels of VEGFR-1 and -2 proteins were determined using Western blot analysis. Amounts of VEGFR-1 and -2 obtained in three independent experiments were quantified and represented as a bar diagram. Levels of VEGFR-1 and -2 in 3AOA- and rhVEGF-A-untreated cells were estimated as 100%. c-d , Cell lysates were immunoprecipitated with anti-phospho-Tyr (anti-p-Tyr). The level of phosphorylated VEGFR-1 and -2 in immunoprecipitates was detected using Western blot analysis with anti-VEGFR-1 and anti-VEGFR-2. Phosphorylation levels of VEGFR-1 and -2 obtained in three independent experiments were quantified and represented as a bar diagram. Phosphorylation levels of VEGFR-1 and -2 in 3AOA- and rhVEGF-A-untreated cells were estimated as 100%. e , HLMECs were serum starved for 6 h, then were treated with different concentrations of 3AOA (0, 2.5, 5 μM) in the presence of rhVEGF-A (20 ng/mL) for 60 min. The phosphorylation levels of FAK, PI3K, AKT, and ERK1/2 were determined using Western blot analysis with anti-p-FAK, anti-p-PI3K, anti-p-AKT, and anti-p-ERK1/2. Data are presented as a mean ± S.D. of three independent experiments ( * p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: Immunoprecipitated proteins were subjected to SDS-PAGE (6%) and Western blotting using mouse anti-VEGFR-1 and goat anti-VEGFR-2 antibodies (Santa Cruz Biotech.

Techniques: Activation Assay, Expressing, Western Blot, Immunoprecipitation

Journal: Gene therapy

Article Title: Gene transfer of RANTES and MCP-1 chemokine antagonists prolongs cardiac allograft survival.

doi: 10.1038/sj.gt.3302765

Figure Lengend Snippet: Figure 1 (a) Immunohistochemical detection of 8ND-Regulated on Activation, Normal T-cell Expressed and Secreted (RANTES) expression by cardiomyocytes in allografts (black nickel-diamino- benzidene (DAB)/H2O2 staining; left: adenovirus (Ad)-Null; right: Ad-8ND-RANTES). (b) Survival curves of grafts receiving Ad-8ND- RANTES (5 1010 or 1010 PFU), Ad-Null or phosphate-buffered saline (PBS) alone. (c) Survival curves of grafts receiving Ad-8ND- monocyte chemoattractant protein (MCP)-1, Ad-8ND-RANTES+ Ad-8ND-MCP-1, Ad-Null (all vectors: 1010 PFU) or PBS. (d) Survival curves of grafts receiving Ad-8ND-RANTES+cyclosporine A (CsA), or CSA alone.

Article Snippet: 8ND-RANTES protein expression was tested by immunostaining using goat anti-human RANTES antibody (C-19; Santa Cruz Biotechnology, sc-1410), followed by biotin-conjugated rabbit anti-goat IgG, StreptABComplex/horseradish peroxidase (HRP) and nickel-diaminobenzidene (DAB)/ H2O2 (Dako).

Techniques: Immunohistochemical staining, Activation Assay, Expressing, Staining, Saline

Journal: Gene therapy

Article Title: Gene transfer of RANTES and MCP-1 chemokine antagonists prolongs cardiac allograft survival.

doi: 10.1038/sj.gt.3302765

Figure Lengend Snippet: Figure 2 Higher panel: Photomicrographs of immunostaining for inflammatory cell infiltrates: adenovirus (Ad)-Null (a–d), Ad-8ND- Regulated on Activation, Normal T-cell Expressed and Secreted (RANTES) (e–h) and Ad-8ND-monocyte chemoattractant protein (MCP)-1 (i–l). ED-1-like cells (a, e, i) are expressed on monocytes/macrophages, cluster of differentiation (CD)4 (b, f, j) on CD4+ lymphocytes and macrophages, CD8a (c, g, k) on cytotoxic T and natural killer (NK) cells, and T-cell receptor (TCR)ab (d, h, l) on TCR-ab-bearing T lymphocytes. Lower panel: Quantitative analysis of leukocyte infiltrates expressed as positive-staining myocardial surface areas, normalized to total myocardial surface areas (horizontal bars are median values).

Article Snippet: 8ND-RANTES protein expression was tested by immunostaining using goat anti-human RANTES antibody (C-19; Santa Cruz Biotechnology, sc-1410), followed by biotin-conjugated rabbit anti-goat IgG, StreptABComplex/horseradish peroxidase (HRP) and nickel-diaminobenzidene (DAB)/ H2O2 (Dako).

Techniques: Immunostaining, Activation Assay, Staining

Journal: Gene therapy

Article Title: Gene transfer of RANTES and MCP-1 chemokine antagonists prolongs cardiac allograft survival.

doi: 10.1038/sj.gt.3302765

Figure Lengend Snippet: Figure 4 Mixed leukocyte reactions in vitro. Lymphocytes were isolated from regional lymph nodes from Lewis (LEW) recipients of F344 donor hearts treated with either adenovirus (Ad)-8ND- Regulated on Activation, Normal T-cell Expressed and Secreted (RANTES) or Ad-Null. They were exposed to F344 donor (white bars) or LEW (black bars) splenocytes (c.p.m.: counts per minute).

Article Snippet: 8ND-RANTES protein expression was tested by immunostaining using goat anti-human RANTES antibody (C-19; Santa Cruz Biotechnology, sc-1410), followed by biotin-conjugated rabbit anti-goat IgG, StreptABComplex/horseradish peroxidase (HRP) and nickel-diaminobenzidene (DAB)/ H2O2 (Dako).

Techniques: In Vitro, Isolation, Activation Assay